Quantitative analysis of gender-regulated transcripts in filarial nematode Brugia malayi by real-time RT-PCR
نویسندگان
چکیده
8 Improved understanding of the biology of reproduction in filarial worms may lead to identification of new targets for drugs or vaccines. Real-time RT-PCR is increasingly being adopted for RNA quantification and genetic analysis. Candidate gender-regulated genes were selected from genes identified in prior studies by differential display RT-PCR and by electronic selection of the Brugia malayi expression sequence tag (EST) database for clusters with possible gender-specific expression (four or more transcripts in male cDNA library ESTs but none in f w g i s e p a b 9
منابع مشابه
Quantitative analysis of gender-regulated transcripts in the filarial nematode Brugia malayi by real-time RT-PCR.
Improved understanding of the biology of reproduction in filarial worms may lead to identification of new targets for drugs or vaccines. Real-time RT-PCR is increasingly being adopted for RNA quantification and genetic analysis. Candidate gender-regulated genes were selected from genes identified in prior studies by differential display RT-PCR and by electronic selection of the Brugia malayi ex...
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Microarray technology permits high-throughput comparisons of gene expression in different parasite stages or sexes and has been used widely. We report the first use of this technology for analysis of gene expression in filarial male and female worms. The slide array (comprised of 65-mer oligos representing 3569 EST clusters) was spotted with sequences selected from the extensive Brugia malayi E...
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85 INTRODUCTION 87 MATERIALS AND METHODS 90 Mosquito Maintenance and Injection Protocol 90 Establishing Brugia Infection 90 Gene Selection & dsRNA Generation 91 Relative Quantitative RT-PCR 93 Quantitative RT-qPCR 95 Phenotype Analysis 98 Statistical Analysis 99 RESULTS 100 Target Genes were Expressed During Mosquito Life Cycle Stages 100 In Squito Suppression of Target Genes was Potent and Spe...
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